Evaluation of a Commercial Microarray System for Detection of SHV-, TEM-, CTX-M-, and KPC-Type β-Lactamase Genes in Gram-Negative Isolates-Reference-Cited by-同舟云学术

Evaluation of a Commercial Microarray System for Detection of SHV-, TEM-, CTX-M-, and KPC-Type β-Lactamase Genes in Gram-Negative Isolates

Published:2010-07 Issue:7 Volume:48 Page:2618-2622
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Endimiani Andrea¹²,Hujer Andrea M.¹²,Hujer Kristine M.¹²,Gatta Julian A.¹,Schriver Andrew C.¹,Jacobs Michael R.³,Rice Louis B.¹,Bonomo Robert A.¹²⁴⁵

Affiliation:

1. Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio

2. Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio

3. Department of Pathology, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio

4. Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio

5. Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio

Abstract

ABSTRACT We evaluated the ability of a commercial microarray system (Check KPC/ESBL; Check-Points Health BV) to detect clinically important class A β-lactamase genes. A total of 106 Gram-negative strains were tested. The following sensitivity and specificity results were recorded, respectively: for bla SHV , 98.8% and 100%; for bla TEM , 100% and 96.4%; and for bla CTX-M and bla KPC , 100% and 100%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00568-10

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