Improved Techniques for Staining and Enumerating Focus Formation in Viral Infectivity Assays-Reference-Cited by-同舟云学术

Improved Techniques for Staining and Enumerating Focus Formation in Viral Infectivity Assays

Published:1974-07 Issue:1 Volume:28 Page:156-157
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Self Donnie J.¹,Pettiss Katherine F.¹,Bigner Darell D.¹

Affiliation:

1. Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710

Abstract

Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.28.1.156-157.1974

Reference5 articles.

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2. Discrete foci of altered chicken embryo cells associated with Rous sarcoma virus in tissue culture;Manaker R. A.;Virology,1956

3. An improved method for enumeration of X-C cell assay for murine leukemia virus;Spahn G. J.;Appl. Microbiol.,1973

4. Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture;Temin H. M.;Virology,1958

5. Vogt P. K. 1969. Focus assay of Rous sarcoma virus. p. 199-211. In K. Habel and N. P. Salzman (ed.) Fundamental techniques in virology. Academic Press Inc. New York.