DNA probes for the identification of Nocardia asteroides

Author:

Brownell G H1,Belcher K E1

Affiliation:

1. Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912.

Abstract

DNA probes for the rapid identification of Nocardia asteroides were obtained by constructing a genomic library of strain GUH-2 in the lambda cloning vector EMBL3. Of 50 recombinant clones tested, 2 were identified that hybridized with 31% of the N. asteroides strains in a reference collection without cross-hybridization with related members of the Actinomycetales. Additional libraries were then generated from selected strains of N. asteroides that had failed to hybridize with any of the GUH-2 clones. Four additional clones were obtained from these strains which, when pooled, provided DNA probes specific for all of the N. asteroides strains tested.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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