Affiliation:
1. Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912.
Abstract
DNA probes for the rapid identification of Nocardia asteroides were obtained by constructing a genomic library of strain GUH-2 in the lambda cloning vector EMBL3. Of 50 recombinant clones tested, 2 were identified that hybridized with 31% of the N. asteroides strains in a reference collection without cross-hybridization with related members of the Actinomycetales. Additional libraries were then generated from selected strains of N. asteroides that had failed to hybridize with any of the GUH-2 clones. Four additional clones were obtained from these strains which, when pooled, provided DNA probes specific for all of the N. asteroides strains tested.
Publisher
American Society for Microbiology
Cited by
12 articles.
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1. Nocardia;Bergey's Manual of Systematics of Archaea and Bacteria;2015-09-14
2. Nocardia and Actinomyces;Molecular Medical Microbiology;2015
3. Phylum XXVI. Actinobacteria phyl. nov.;Bergey’s Manual® of Systematic Bacteriology;2012
4. Identification phénotypique et moléculaire des bactéries appartenant au genre Nocardia;Revue Francophone des Laboratoires;2007-04
5. Clinical and Laboratory Features of the
Nocardia
spp. Based on Current Molecular Taxonomy;Clinical Microbiology Reviews;2006-04