Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity-Reference-Cited by-同舟云学术

Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity

Published:2004-07-15 Issue:14 Volume:186 Page:4802-4807
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Kuban Wojciech¹,Jonczyk Piotr¹,Gawel Damian¹²,Malanowska Karolina¹,Schaaper Roel M.²,Fijalkowska Iwona J.¹

Affiliation:

1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland

2. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Abstract

ABSTRACT We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli . We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F′( pro-lac ) episome.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.186.14.4802-4807.2004

Reference41 articles.

1. Bebenek, K., and T. A. Kunkel. 2000. Streisinger revisited: DNA synthesis errors mediated by substrate misalignments. Cold Spring Harbor Symp. Quant. Biol. 65 : 81-91.

2. Escherichia coli DNA Polymerase III Can Replicate Efficiently past a T-T cis-syn Cyclobutane Dimer if DNA Polymerase V and the 3′ to 5′ Exonuclease Proofreading Function Encoded by dnaQ Are Inactivated

3. Brotcorne-Lannoye, A., and G. Maenhaut-Michel. 1986. Role of RecA protein in untargeted UV mutagenesis of bacteriophage λ: evidence for the requirement for the dinB gene. Proc. Natl. Acad. Sci. USA 83 : 3904-3908.

4. Cupples, C. G., M. Cabrera, C. Cruz, and J. H. Miller. 1990. A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations. Genetics 125 : 275-280.

5. Cupples, C. G., and J. H. Miller. 1989. A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions. Proc. Natl. Acad. Sci. USA 86 : 5345-5349.

Cited by 62 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. “The Translesion Polymerase Pol Y1 is a Constitutive Component of theB. subtilisReplication Machinery”;2023-10-06

2. Focus and persistence: how Pol IV unblocks stalled DNA synthesis;Nature Structural & Molecular Biology;2022-09

3. Suppressing evolution of antibiotic resistance through environmental switching;Theoretical Ecology;2022-02-10

4. Suppressing evolution through environmental switching;2021-02-11

5. Antibiotic-Induced Mutagenesis: Under the Microscope;Frontiers in Microbiology;2020-10-22