Diagnosing Pulmonary Tuberculosis by Using Sequence-Specific Purification of Urine Cell-Free DNA-Reference-Cited by-同舟云学术

Diagnosing Pulmonary Tuberculosis by Using Sequence-Specific Purification of Urine Cell-Free DNA

Published:2021-07-19 Issue:8 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Oreskovic Amy¹^ORCID,Panpradist Nuttada¹,Marangu Diana²,Ngwane M. William³,Magcaba Zanele P.³,Ngcobo Sindiswa³,Ngcobo Zinhle³,Horne David J.⁴⁵,Wilson Douglas P. K.³⁶,Shapiro Adrienne E.⁴⁵,Drain Paul K.⁴⁵^ORCID,Lutz Barry R.¹

Affiliation:

1. Department of Bioengineering, University of Washington, Seattle, Washington, USA

2. Department of Paediatrics and Child Health, University of Nairobi, Nairobi, Kenya

3. Umkhuseli Innovation and Research Management, Pietermaritzburg, South Africa

4. Department of Medicine, University of Washington, Seattle, Washington, USA

5. Department of Global Health, University of Washington, Seattle, Washington, USA

6. Edendale Hospital, University of KwaZulu-Natal, Pietermaritzburg, South Africa

Abstract

Transrenal urine cell-free DNA (cfDNA) is a promising tuberculosis (TB) biomarker, but is challenging to detect because of the short length (<100 bp) and low concentration of TB-specific fragments. We aimed to improve the diagnostic sensitivity of TB urine cfDNA by increasing recovery of short fragments during sample preparation.

Funder

Bill and Melinda Gates Foundation

HHS | NIH | National Institute of Allergy and Infectious Diseases

National Science Foundation

UW | Center for AIDS Research, University of Washington

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00074-21

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