Simple technique for separation of concentrated immunoglobulin M fractions by multi-sample gel chromatography and its application to rubella serodiagnosis

Abstract

A simple technique has been developed with which five serum specimens can be chromatographed at one time and concentrated immunoglobulin M fractions can be obtained without using fraction collectors and ultraviolet monitors. When applied to the detection of rubella immunoglobulin M antibody, this technique gave results comparable to the sucrose gradient centrifugation method.