Automated Microtitration Test for Antistreptolysin O

Author:

Dixon J. M. S.1,Grocholski J. J.1

Affiliation:

1. Provincial Laboratory of Public Health, The University of Alberta, Edmonton, Alberta, Canada

Abstract

A commercially available instrument that automatically makes serial dilutions and delivers reagents was used for the determination of antistreptolysin O titers in serum. The automated method was compared with tube-dilution and manual microtitration techniques. It gave higher reproducibility of results and was quicker to perform than both the other tests; and it was much more economical in reagents than the tube test. The automated technique is considered to be the best of the three methods when more than a small number of specimens are examined at one time. It is now in routine use in our laboratory.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference5 articles.

1. A comparison of two methods of estimating antistreptolysin 0;Brighton W. D.;Mon. Bull. Min. Health Lab. Serv. Directed Med. Res. Counc.,1967

2. Protocol for micro antistreptolysin 0 determinations;Edwards E. A.;J. Bacteriol.,1964

3. Evaluation of an automatic diluting device for microbiological applications;Goss W. A.;Appl. Microbiol.,1968

4. Micro antistreptolysin 0 test;Klein G. C.;Appl. Microbiol.,1968

5. A modification of the technic for determination of the antistreptolysin titer;Rantz L. A.;Proc. Soc. Exp. Biol. Med.,1945

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