Assessment of SARS-CoV-2 Genome Sequencing: Quality Criteria and Low-Frequency Variants-Reference-Cited by-同舟云学术

Assessment of SARS-CoV-2 Genome Sequencing: Quality Criteria and Low-Frequency Variants

Published:2021-09-20 Issue:10 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Jacot Damien¹,Pillonel Trestan¹,Greub Gilbert¹,Bertelli Claire¹^ORCID

Affiliation:

1. Institute of Microbiology, Laboratory of Genomics and Metagenomics, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland

Abstract

Although many laboratories worldwide have developed their sequencing capacities in response to the need for SARS-CoV-2 genome-based surveillance of variants, only a few reported some quality criteria to ensure sequence quality before lineage assignment and submission to public databases. Hence, we aimed here to provide simple quality control criteria for SARS-CoV-2 sequencing to prevent erroneous interpretation of low-quality or contaminated data.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00944-21

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