Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism

Author:

Frénay H M1,Theelen J P1,Schouls L M1,Vandenbroucke-Grauls C M1,Verhoef J1,van Leeuwen W J1,Mooi F R1

Affiliation:

1. Molecular Microbiology Unit, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

Abstract

The X region of the protein A gene of Staphylococcus aureus contains a highly polymorphic sequence which is composed of repeats of 24 bp. We used amplification by PCR to investigate whether this region could be used to discriminate between epidemic and nonepidemic methicillin-resistant S. aureus (MRSA) strains. Most epidemic MRSA strains (24 of 33) harbored more than seven repeats, while most nonepidemic MRSA strains (10 of 14) contained seven or fewer repeats. It is conceivable that a longer X region results in a better exposition of the Fc-binding region of protein A, thereby facilitating colonization of host surfaces and contributing to the epidemic phenotype.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

1. Molecular organization of the protein A gene and its expression in recombinant host organisms;Colbert D.;J. Biol. Response Modif.,1984

2. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms;Goh S.;J. Clin. Microbiol.,1992

3. Region X, the cell-wall-attachment part of staphylococcal protein A;Guss B.;Eur. J. Biochem.,1984

4. Revised guidelines for the control of epidemic methicillin-resistant Staphylococcus aureus;Hospital Infection Society and British Society for Antimicrobial Chemotherapy.;J. Hosp. Infect.,1990

5. Marples , R. R. , and S. Reith . Methicillin-resistant Staphylococcus aureus in England and Wales. 1992 . Commun. Dis. Rep. 2 : 25 - 29 .

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