Affiliation:
1. Molecular Microbiology Unit, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Abstract
The X region of the protein A gene of Staphylococcus aureus contains a highly polymorphic sequence which is composed of repeats of 24 bp. We used amplification by PCR to investigate whether this region could be used to discriminate between epidemic and nonepidemic methicillin-resistant S. aureus (MRSA) strains. Most epidemic MRSA strains (24 of 33) harbored more than seven repeats, while most nonepidemic MRSA strains (10 of 14) contained seven or fewer repeats. It is conceivable that a longer X region results in a better exposition of the Fc-binding region of protein A, thereby facilitating colonization of host surfaces and contributing to the epidemic phenotype.
Publisher
American Society for Microbiology
Reference10 articles.
1. Molecular organization of the protein A gene and its expression in recombinant host organisms;Colbert D.;J. Biol. Response Modif.,1984
2. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms;Goh S.;J. Clin. Microbiol.,1992
3. Region X, the cell-wall-attachment part of staphylococcal protein A;Guss B.;Eur. J. Biochem.,1984
4. Revised guidelines for the control of epidemic methicillin-resistant Staphylococcus aureus;Hospital Infection Society and British Society for Antimicrobial Chemotherapy.;J. Hosp. Infect.,1990
5. Marples , R. R. , and S. Reith . Methicillin-resistant Staphylococcus aureus in England and Wales. 1992 . Commun. Dis. Rep. 2 : 25 - 29 .
Cited by
116 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献