Perturbing HIV-1 Ribosomal Frameshifting Frequency Reveals a cis Preference for Gag-Pol Incorporation into Assembling Virions-Reference-Cited by-同舟云学术

Perturbing HIV-1 Ribosomal Frameshifting Frequency Reveals a cis Preference for Gag-Pol Incorporation into Assembling Virions

Published:2022-01-12 Issue:1 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Benner Bayleigh E.¹²^ORCID,Bruce James W.¹³,Kentala Jacob R.¹,Murray Magdalena¹,Becker Jordan T.¹^ORCID,Garcia-Miranda Pablo¹⁴,Ahlquist Paul¹³,Butcher Samuel E.⁴,Sherer Nathan M.¹^ORCID

Affiliation:

1. Department of Oncology (McArdle Laboratory for Cancer Research), Institute for Molecular Virology, and Carbone Cancer Center, University of Wisconsin—Madison, Madison, Wisconsin, USA

2. UW—Madison Microbiology Doctoral Training Program, Madison, Wisconsin, USA

3. John and Jeanne Rowe Center for Research in Virology, Morgridge Institute for Research, Madison, Wisconsin, USA

4. Department of Biochemistry, University of Wisconsin—Madison, Madison, Wisconsin, USA

Abstract

HIV-1 infectivity requires incorporation of the Gag-Pol (GP) precursor polyprotein into virions during the process of virus particle assembly. Mechanisms dictating GP incorporation into assembling virions are poorly defined, with GP levels in virions traditionally thought to solely reflect relative levels of Gag and GP expressed in cells, dictated by the frequency of a −1 programmed ribosomal frameshifting (PRF) event that occurs in gag-pol mRNAs.

Funder

Greater Milwaukee Foundation

HHS | National Institutes of Health

National Science Foundation

University of Wisconsin-Madison

Wisconsin Alumni Research Foundation

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01349-21