Characterization by Multilocus Enzyme Electrophoresis of Listeria monocytogenes Isolates Involved in Ovine Listeriosis Outbreaks in Scotland from 1989 to 1991

Author:

Baxter F.1,Wright F.1,Chalmers R. M.1,Low J. C.1,Donachie W.1

Affiliation:

1. Scottish Agricultural College Veterinary Services, Greycrook, St. Boswells TD6 0EU, Scottish Agricultural Statistics Service, University of Edinburgh, Edinburgh EH9 3JZ, 2 Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH, 3 and Scottish Agricultural College Veterinary Services, Edinburgh EH26 0QE, 4 United Kingdom

Abstract

Initial results from a study of five small ovine listeriosis outbreaks in Scotland in 1989 to 1991 are presented. Forty-eight isolates including three from silage were typed at 10 polymorphic enzyme loci by using multilocus enzyme electrophoresis resulting in the identification of 12 electrophoretic types. Phylogenetic analysis partitioned the 12 electrophoretic types into two statistically distinct divisions distinguishing 1/2a serotypes from non-1/2a serotypes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference27 articles.

1. Anonymous. 1988. Veterinary investigation diagnosis analysis II. Central Veterinary Laboratory Ministry of Agriculture Fisheries and Food Weybridge England.

2. A phage typing system for Listeria monocytogenes and its use in epidemiological studies;Audurier A.;Clin. Invest. Med.,1984

3. Analysis of clinical and foodborne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations;Bibb W. F.;Appl. Environ. Microbiol.,1990

4. Analysis of Listeria monocytogenes by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations;Bibb W. F.;Int. J. Food Microbiol.,1989

5. Bille J. 1990. Epidemiology of human listeriosis in Europe with special reference to the Swiss outbreak p. 71-74. In A. J. Miller J. L. Smith and G. A. Somkuti (ed.) Foodborne listeriosis. Elsevier Science Publishing Inc. New York.

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