Bacteriophage Lambda as a Delivery Vector for Tn 10 -Derived Transposons in Xenorhabdus bovienii-Reference-Cited by-同舟云学术

Bacteriophage Lambda as a Delivery Vector for Tn 10 -Derived Transposons in Xenorhabdus bovienii

Published:1993-09 Issue:9 Volume:59 Page:3050-3055
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Francis Matthew S.¹,Parker Angela F.¹,Morona Renato¹,Thomas Connor J.¹

Affiliation:

1. Department of Microbiology and Immunology, University of Adelaide, G.P.O. Box 498, Adelaide, South Australia, Australia, 5001

Abstract

Xenorhabdus bovienii wild-type strains lack a functional receptor protein (LamB) in the outer membrane and as a result are unable to adsorb coliphage lambda (λ). Introduction of plasmids encoding lamB into X. bovienii T228 results in constitutive expression of LamB in the outer membrane of this organism. LamB-expressing strains of X. bovienii adsorb lambda bacteriophage particles and can be used as hosts for lambda::Tn constructs. A Tn 10 -derived transposon, element 9 (J. C. Way, D. Davis, D. Morisato, D. E. Roberts, and N. Kleckner, Gene 32:369-379, 1984) was used to construct a variety of insertion mutants of X. bovienii. Mutants that had altered expression of protease, lipase, DNase, dye-binding capability, and hemolytic activity, in addition to a series of auxotrophic mutants, were isolated.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.59.9.3050-3055.1993

Reference34 articles.

1. Cell-cell interactions in conjugating Escherichia coli: Con- mutants and stabilization of mating aggregates;Achtman M.;Proc. Natl. Acad. Sci. USA,1978

2. Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoplectana and Heterorhabditis;Akhurst R. J.;J. Gen. Microbiol.,1980

3. Antibiotic activity of Xenorhabdus spp. bacteria symbiotically associated with insect pathogenic nematodes of the families Heterorhabditidae and Steinernematidae;Akhurst R. J.;J. Gen. Microbiol.,1982

4. Taxonomic study ofXenorhabdus, a genus of bacteria symbiotically associated with insect pathogenic nematodes;Akhurst R. J.;Int. J. Syst. Bacteriol.,1983

5. Specificity of the association between Steinernema spp. (Nematoda: Steinernematidae) and Xenorhabdus spp. (Eubacteriales: Enterobacteriaceae);Akhurst R. J.;Exp. Parasitol.,1983

Cited by 10 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Xenorhabdus;Bergey's Manual of Systematics of Archaea and Bacteria;2015-09-14

2. The Genera Photorhabdus and Xenorhabdus;The Prokaryotes;2006

3. 4 Isolation and Development of Transposons;Methods in Microbiology;1999

4. Survival of Xenorhabdus nematophilus and Photorhabdus luminescens in water and soil;Journal of Applied Microbiology;1997-12

5. XENORHABDUS AND PHOTORHABDUS SPP.: Bugs That Kill Bugs;Annual Review of Microbiology;1997-10