Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis-Reference-Cited by-同舟云学术

Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis

Published:1993-09 Issue:9 Volume:59 Page:3141-3144
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Harsono K D¹,Kaspar C W¹,Luchansky J B¹

Affiliation:

1. Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.

Abstract

Genomic DNAs of Escherichia coli O157:H7 strains isolated from patients and food samples were analyzed by pulsed-field gel electrophoresis. The rare-cutting endonucleases SfiI and XbaI generated 6 and 10 distinct genomic profiles, respectively, for the 22 strains analyzed, indicating that this technique may find application for epidemiologic studies. Summation of XbaI fragments from five E. coli O157:H7 strains estimated the genomic length at ca. 4.7 Mb.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.59.9.3141-3144.1993

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