Enhanced Tracking of Nosocomial Transmission of Endemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Type IV Isolates among Patients and Environmental Sites by Use of Whole-Genome Sequencing-Reference-Cited by-同舟云学术

Enhanced Tracking of Nosocomial Transmission of Endemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Type IV Isolates among Patients and Environmental Sites by Use of Whole-Genome Sequencing

Published:2016-02 Issue:2 Volume:54 Page:445-448
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kinnevey Peter M.¹,Shore Anna C.¹²,Mac Aogáin Micheál²,Creamer Eilish³,Brennan Gráinne I.¹⁴,Humphreys Hilary³⁵,Rogers Thomas R.²,O'Connell Brian²⁴,Coleman David C.¹^ORCID

Affiliation:

1. Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland

2. Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland

3. Department of Microbiology, Beaumont Hospital, Dublin, Ireland

4. National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland

5. Department of Clinical Microbiology, Royal College of Surgeons in Ireland Education and Research Centre, Beaumont Hospital, Dublin, Ireland

Abstract

ABSTRACT Whole-genome sequencing (WGS) of 41 patient and environmental sequence type 22 methicillin-resistant Staphylococcus aureus staphylococcal cassette chromosome mec type IV (ST22-MRSA-IV) isolates recovered over 6 weeks in one acute hospital ward in Dublin, Ireland, where ST22-MRSA IV is endemic, revealed 228 pairwise combinations differing by <40 single nucleotide variants corresponding to potential cross-transmission events (CTEs). In contrast, 15 pairwise combinations of isolates representing five CTEs were previously identified by conventional molecular epidemiological typing. WGS enhanced ST22-MRSA-IV tracking and highlighted potential transmission of MRSA via the hospital environment.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02662-15

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