Development of a polymerase chain reaction-probe test for identification of Alloiococcus otitis-Reference-Cited by-同舟云学术

Development of a polymerase chain reaction-probe test for identification of Alloiococcus otitis

Published:1992-08 Issue:8 Volume:30 Page:2177-2180
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Aguirre M¹,Collins M D¹

Affiliation:

1. Department of Microbiology, Agriculture and Food Research Council Institute of Food Research, Reading Laboratory, United Kingdom.

Abstract

A rapid polymerase chain reaction test was developed for specific identification of the human middle ear pathogen Alloiococcus otitis. Primers for the enzymatic amplification reaction were designed from highly specific sequences within the 16S rRNA gene. In addition, a confirmatory test based on hybridization of the polymerase chain reaction products to a specific internal probe was developed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.30.8.2177-2180.1992

Reference12 articles.

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3. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli;Brosius J.;Proc. Natl. Acad. Sci. USA,1978

4. Recovery of a unique bacterial organism in human middle ear fluid and its possible role in chronic otitis media;Faden H.;J. Clin. Microbiol.,1989

5. Streptococcus raffinolactis (Orla-Jensen and Hansen); a group N streptococcus found in raw milk;Garvie E. I.;Int. J. Syst. Bacteriol.,1978

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