Immobilization of Aspergillus beta-glucosidase on chitosan

Author:

Bissett F,Sternberg D

Abstract

beta-Glucosidase of Aspergillus phoenicis QM 329 was immobilized on chitosan, using the bifunctional agent glutaraldehyde. The most active preparation based on the amount of support contained a 1:2.5 enzyme-to-chitosan ratio (wt/wt). However, the specific activity of the bound enzyme decreased from 10 to 1% with increasing enzyme-to-chitosan ratio. Compared with free beta-glucosidase, the immobilized enzyme exhibited: (i) a similar pH optimum but more activity at lower pH values; (ii) improved thermal stability; (iii) a similar response to inhibition by glucose; and (iv) mass transfer limitations as reflected by higher apparent Km and lower energy of activation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference15 articles.

1. 8-D-glucosidase chemically bound to microcrystalline cellulose;Barker S. A.;Carbohydr. Res.,1971

2. Bernath F. R. and W. R. Vieth. 1974. Collagen as a carrier for enzymes: materials science and process engineering aspects of enzyme engineering p. 157-185. In A. C. Olson and C. L. Cooney (ed.) Immobilized enzymes in food and microbial processes. Plenum Press New York.

3. Characteristics of yeast invertase immobilized on porous cellulose beads;Dickensheets P. A.;Biotechnol. Bioeng.,1977

4. Cellobiase from Trichoderma viride: purification, properties, kinetics, and mechanism;Gong D.;Biotechnol. Bioeng.,1977

5. Halliwell G. 1975. Action of the cellulase complex p. 319-336. In M. Bailey T.-M. Enari and M. Linko (ed.) Symposium on the Enzymatic Hydrolysis of Cellulose. The Finnish National Fund for Research and Development Helsinki.

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