Glycolipids from some extreme thermophilic bacteria belonging to the genus Thermus

Abstract

The lipids of Thermus aquaticus YT1, Thermus thermophilus HB8, Thermus sp. strains H and J (from Icelandic hot springs), and Thermus sp. strain NH (from domestic hot water) have been investigated. Each strain contained two major components, a glycolipid and a glycophospholipid, which have been isolated and analyzed. All of the strains contained as the principal component (41 to 57% of total lipid) a diacyldiglycosyl-(N-acyl)glycosaminylglucosylglycerol, but the five glycolipids differed in carbohydrate composition. The glycophospholipid appeared to be identical in each strain and contained an N-acylglucosamine residue. The principal fatty acids were C15 and C17 branched-chain compounds. This unique polar lipid composition should be of value in the classification of other thermophiles in the genus Thermus. The exceptionally high carbohydrate content of the lipids of these extreme thermophiles may be of significance in relation to the molecular basis of thermophily.