Rapid and overnight microdilution antibiotic susceptibility testing with the Sensititre Breakpoint Autoreader system

Abstract

The Sensititre Breakpoint Autoreader system (SBAS) is a broth microdilution method with one to three concentrations of each antibiotic and innovative fluorescence technology to define inhibitory endpoints. We tested 248 gram-negative bacilli and 80 gram-positive cocci using both the rapid (5 h) and overnight (18 h) SBAS procedures. Inhibitory endpoints were also determined by visual inspection of the microdilution trays after 18 h of incubation. SBAS results were compared with those obtained by a standardized disk diffusion (SDD) procedure. Agreement between the rapid SBAS and SDD results for all antibiotic-organism combinations was found in 3,730 of 4,571 (81.6%) tests, with 3.9% very major, 6.5% major, and 7.9% minor discrepancies noted. Data analysis by organism group revealed 86.8, 57.3, 71.4, and 62.3% agreement for members of the family Enterobacteriaceae, Pseudomonas spp., staphylococci, and enterococci, respectively. The results of the overnight SBAS and SDD agreed in 4,154 of 4,654 (89.2%) tests, with 2.3% very major, 1.3% major, and 7.1% minor discrepancies recorded. Concordance was noted in 90.4, 78.1, 90.6, and 83.3% of the comparisons for the members of the Enterobacteriaceae, Pseudomonas spp., staphylococci, and enterococci, respectively. The inhibitory endpoints determined with the Autoreader were as reliable as those determined by visual inspection after 18 h of incubation.