Nef-Induced Major Histocompatibility Complex Class I Down-Regulation Is Functionally Dissociated from Its Virion Incorporation, Enhancement of Viral Infectivity, and CD4 Down-Regulation-Reference-Cited by-同舟云学术

Nef-Induced Major Histocompatibility Complex Class I Down-Regulation Is Functionally Dissociated from Its Virion Incorporation, Enhancement of Viral Infectivity, and CD4 Down-Regulation

Published:2000-03-15 Issue:6 Volume:74 Page:2907-2912
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Akari Hirofumi¹,Arold Stefan¹,Fukumori Tomoharu¹,Okazaki Toshiyuki¹,Strebel Klaus²,Adachi Akio¹

Affiliation:

1. Department of Virology, The University of Tokushima School of Medicine, Tokushima, Tokushima 770-8503, Japan,1 and

2. Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-04402

Abstract

ABSTRACT The N-terminal alpha-helix domain of the human immunodeficiency virus type 1 (HIV-1) Nef protein plays important roles in enhancement of viral infectivity, virion incorporation of Nef, and the down-regulation of major histocompatibility complex class I (MHC-I) expression on cell surfaces. In this study, we demonstrated that Met 20 in the alpha-helix domain was indispensable for the ability of Nef to modulate MHC-I expression but not for other events. We also showed that Met 20 was unnecessary for the down-regulation of CD4. These findings indicate that the region governing MHC-I down-regulation is proximate in the alpha-helix domain but is dissociated functionally from that determining enhancement of viral infectivity, virion incorporation of Nef, and CD4 down-regulation.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.74.6.2907-2912.2000

Reference33 articles.

1. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

2. Generation and characterization of the human immunodeficiency virus type 1 mutants;Adachi A.;Arch. Virol.,1991

3. Mutational analysis of HIV-1 Nef: identification of two mutants that are temperature-sensitive for CD4 downregulation;Aiken C.;Virology,1996

4. Pseudotyping human immunodeficiency virus type 1 by vesicular stomatitis virus G protein does not reduce the cell-dependent requirement of Vif for optimal infectivity: functional difference between Vif and Nef;Akari H.;J. Gen. Virol.,1999

5. Solution structure of a polypeptide from the N terminus of the HIV protein Nef;Barnham K. J.;Biochemistry,1997

Cited by 100 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Recognition of HIV-1-infected fibrocytes lacking Nef-mediated HLA-B downregulation by HIV-1-specific T cells;Journal of Virology;2024-07-23

2. Chimeric antigen receptors enable superior control of HIV replication by rapidly killing infected cells;PLOS Pathogens;2023-12-15

3. Impaired ability of Nef to counteract SERINC5 is associated with reduced plasma viremia in HIV-infected individuals;Scientific Reports;2020-11-10

4. Multifunctional Roles of the N-Terminal Region of HIV-1 SF2 Nef Are Mediated by Three Independent Protein Interaction Sites;Journal of Virology;2019-12-12

5. The autophagy protein ATG9A promotes HIV-1 infectivity;Retrovirology;2019-07-03