Assessment of Clofazimine and TB47 Combination Activity against Mycobacterium abscessus Using a Bioluminescent Approach

Author:

Liu Yang1,Tan Yaoju2,Islam M. Mahmudul13,Cao Yuanyuan1,Lu Xiaoyun4,Zeng Sheng1,Hameed H. M. Adnan13,Zhou Peipei1,Cai Xingshan2,Wang Shuai13,Mugweru Julius N.135,Zhang Guoliang6,Yin Huancai7,Liu Jianxiong2,Nuermberger Eric8,Zhang Tianyu13ORCID

Affiliation:

1. State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China

2. State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou, China

3. University of Chinese Academy of Sciences, Beijing, China

4. School of Pharmacy, Jinan University, Guangzhou, China

5. Department of Biological Sciences, University of Embu, Embu, Kenya

6. Guangdong Key Laboratory of Emerging Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, Shenzhen, China

7. Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Jiangsu, China

8. Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University, Baltimore, Maryland, USA

Abstract

Mycobacterium abscessus is intrinsically resistant to most antimicrobial agents. The emerging infections caused by M. abscessus and the lack of effective treatment call for rapid attention. Here, we intended to construct a selectable marker-free autoluminescent M. abscessus strain (designated UAlMab) as a real-time reporter strain to facilitate the discovery of effective drugs and regimens for treating M. abscessus . The UAlMab strain was constructed using the dif /Xer recombinase system.

Funder

National Mega-project of China for Innovative Drugs

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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