Conversion of Sphingobium chlorophenolicum ATCC 39723 to a Hexachlorobenzene Degrader by Metabolic Engineering-Reference-Cited by-同舟云学术

Conversion of Sphingobium chlorophenolicum ATCC 39723 to a Hexachlorobenzene Degrader by Metabolic Engineering

Published:2006-03 Issue:3 Volume:72 Page:2283-2286
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yan Da-Zhong¹²,Liu Hong¹,Zhou Ning-Yi¹

Affiliation:

1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

2. Graduate School, Chinese Academy of Sciences, Beijing 100039, China

Abstract

ABSTRACT The gene cassette ( camA + camB + camC ) encoding a cytochrome P-450 cam variant was integrated into the nonessential gene pcpM of the pentachlorophenol degrader Sphingobium chlorophenolicum ATCC 39723 by homologous recombination. The recombinant strain could degrade hexachlorobenzene at a rate of 0.67 nmol · mg (dry weight) −1 · h −1 , and intermediate pentachlorophenol was also identified.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.3.2283-2286.2006

Reference14 articles.

1. Barber, J. L., A. J. Sweetman, D. van Wijk, and K. C. Jones. 2005. Hexachlorobenzene in the global environment: emissions, levels, distribution, trends and processes. Sci. Total Environ.349:1-44.

2. Organization and Regulation of Pentachlorophenol-Degrading Genes in Sphingobium chlorophenolicum ATCC 39723

3. Chang, B. V., C. J. Su, and S. Y. Yuan. 1998. Microbial hexachlorobenzene dechlorination under three reducing conditions. Chemosphere36:2721-2730.

4. Chen, X., A. Christopher, L. P. Jones, S. G. Bell, Q. Guo, F. Xu, Z. Rao, and L.-L. Wong. 2002. Crystal structure of the F87W/Y96F/V247L mutant of cytochrome P-450cam with 1,3,5-trichlorobenzene bound and further protein engineering for the oxidation of pentachlorobenzene and hexachlorobenzene. J. Biol. Chem.277:37519-37526.

5. Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes

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