Optimization and Clinical Evaluation of an Automated Commercial Analyte-Specific Reagent Assay for <i>Mycoplasmoides genitalium</i> Macrolide Resistance Detection in Primary Clinical Specimens-Reference-Cited by-同舟云学术

Optimization and Clinical Evaluation of an Automated Commercial Analyte-Specific Reagent Assay for Mycoplasmoides genitalium Macrolide Resistance Detection in Primary Clinical Specimens

Published:2023-07-20 Issue:7 Volume:61 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Munson Erik¹²^ORCID,Zapp Amanda¹,Moore Josephine¹,Lavey Stephen C.³,Russell Hunter¹,Munson Kimber L.⁴,Martin Irene⁵

Affiliation:

1. Department of Medical Laboratory Science, Marquette University, Milwaukee, Wisconsin, USA

2. Wisconsin Clinical Laboratory Network Laboratory Technical Advisory Group, Madison, Wisconsin, USA

3. Carleton College, Northfield, Minnesota, USA

4. Ascension Wisconsin, Milwaukee, Wisconsin, USA

5. National Microbiology Laboratory Public Health Agency of Canada, Winnipeg, Canada

Abstract

With improvement in laboratory diagnosis of Mycoplasmoides genitalium infection through molecular diagnostics, macrolide resistance determination within M. genitalium -positive patients is necessary. In this study, we report baseline parameters for an analyte-specific reagent (ASR) macrolide resistance real-time reverse transcriptase PCR on an open access analyzer and evaluated detection of macrolide resistance-mediated mutation (MRM) within 23S rRNA in a clinical specimen set.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.00335-23

Reference48 articles.

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3. Microbiological and serological study of non-gonococcal urethritis with special reference to Mycoplasma genitalium.

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5. Mycoplasma genitalium Nonadherent Phase Variants Arise by Multiple Mechanisms and Escape Antibody-Dependent Growth Inhibition

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