Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system

Author:

Krech T1,Bleckmann M1,Paatz R1

Affiliation:

1. Institute for Medical Microbiology and Virology, University of Düsseldorf, Federal Republic of Germany.

Abstract

A total of 1,229 urogenital samples of patients with sexually transmitted diseases and prostitutes were inoculated simultaneously in McCoy cells and Buffalo green monkey (BGM) cells by using a microtitration technique. BGM cells proved to be slightly more sensitive than McCoy cells, and they usually showed more and larger inclusions and were more resistant to cytotoxic substances in samples. The decrease in sensitivity as a result of mycoplasmal contamination was much more prominent in McCoy cells than in BGM cells.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Vergleichende Untersuchungen an Buffalo Green Monkey (BGM)-Zellen und Mausen zur Isolierung von Chlamydia psittaci aus Kot- und Organproben von Vogeln;Arens M.;Zentralbl. Veterinaermed. Reihe B,1981

2. Hobson D. N. Lee E. Quayle and E. E. Beckett. 1982. Growth of Chlamydia trachomatis in Buffalo green monkey cells. Lancet ii:872-873.

3. Effect of blind passage and multiple sampling on recovery of Chlamydia trachomatis from urogenital specimens;Jones R. B.;J. Clin. Microbiol.,1986

4. Kenny G. E. 1985. Mycoplasma contamination of cell cultures p. 210-227. In I. Gylsdorff (ed.) Infektionen durch Mycoplasmatales. Ferdinand Enke Verlag Stuttgart Federal Republic of Germany.

5. Cultural method for large-scale screening for Chlamydia trachomatis genital infection;Mallinson H.;J. Clin. Pathol.,1981

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