Spin-Labeling Studies on the Membrane of a Facultative Thermophilic Bacillus

Author:

Chan May1,Virmani Y. P.1,Himes R. H.1,Akagi J. M.1

Affiliation:

1. Departments of Microbiology and Biochemistry, University of Kansas, Lawrence, Kansas 66044

Abstract

Spin-labeling studies were conducted to elucidate the rigidity and viscosity of the membrane of a facultative thermophilic bacillus. By employing nitroxide stearate probes, it was observed that this fatty acid was incorporated into the membrane fraction. Electron spin resonance spectroscopy indicated that cells grown at 55 C contained a membrane which was more rigid and viscous than the membrane from cells grown at 37 C.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference18 articles.

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3. A simple method for the isolation and purification of total lipids from animal tissues;Folch J.;J. Biol. Chem.,1957

4. Nitroxide free radicals: spin labels for probing biomolecular structure;Griffith 0.;AqZounts Chem. Res.,1969

5. Hamilton C. L. and H. M. McConnell. 1968. Spin labels p. 115-149. In A. Rich and N. Davidson (ed.) Structural chemistry and molecular biology. W. H. Freeman and Co. San Francisco.

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