Usefulness of Leifson Staining Method in Diagnosis of Helicobacter pylori Infection

Author:

Piccolomini Raffaele1,Di Bonaventura Giovanni1,Neri Matteo2,Di Girolamo Arturo3,Catamo Giovanni1,Pizzigallo Eligio3

Affiliation:

1. Department of Biomedical Sciences, Section of Clinical Microbiology,1 and

2. Department of Medicine and Aging Sciences, Section of Gastroenterology2 and

3. Section of Infectious Diseases,3 “G. D’Annunzio” University, Chieti, Italy

Abstract

ABSTRACT The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) ( P < 0.005). Leifson staining is a sensitive, rapid, economical method for diagnosis of H. pylori infection in dyspeptic patients.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference24 articles.

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2. A Simplified Leifson Flagella Stain

3. Touch cytology, a useful diagnostic method for diagnosis of upper gastrointestinal tract infection;Debongnie J. C.;Dig. Dis. Sci.,1989

4. Cytology: a simple, rapid, sensitive method in the diagnosis of Helicobacter pylori;Debongnie J. C.;Am. J. Gastroenterol.,1992

5. Identification of Campylobacter pylori in gastric biopsy smears;Faverly D.;Acta Cytol.,1990

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