Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription-Reference-Cited by-同舟云学术

Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription

Published:2001-07-15 Issue:14 Volume:75 Page:6676-6681
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Thiel Volker¹,Herold Jens¹,Schelle Barbara¹,Siddell Stuart G.¹

Affiliation:

1. Institute of Virology and Immunology, University of Würzburg, 97078 Würzburg, Germany

Abstract

ABSTRACT We have used vaccinia virus as a vector to clone a 22.5-kbp cDNA that represents the 5′ and 3′ ends of the human coronavirus 229E (HCoV 229E) genome, the HCoV 229E replicase gene, and a single reporter gene (coding for green fluorescent protein [GFP]) located downstream of a regulatory element for coronavirus mRNA transcription. When RNA transcribed from this cDNA was transfected into BHK-21 cells, a small percentage of cells displayed strong fluorescence. A region of the mRNA encoding GFP was amplified by PCR and shown to have the unique mRNA leader-body junction indicative of coronavirus-mediated transcription. These data show that the coronavirus replicase gene products suffice for discontinuous subgenomic mRNA transcription.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.75.14.6676-6681.2001

Reference31 articles.

1. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression;Agapov E. V.;Proc. Natl. Acad. Sci. USA,1998

2. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome;Almázan F.;Proc. Natl. Acad. Sci. USA,2000

3. Ausubel F. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology. 1987 John Wiley & Sons New York N.Y

4. Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription

5. The genome organization of the Nidovirales: similarities and differences between arteri-, toro-, and coronaviruses;de Vries A. F.;Semin. Virol.,1997

Cited by 129 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Novel Pan-Coronavirus 3CL Protease Inhibitor MK-7845: Biological and Pharmacological Profiling;Viruses;2024-07-18

2. Identification of two flavonoids antiviral inhibitors targeting 3C-like protease of porcine epidemic diarrhea virus;Frontiers in Microbiology;2024-03-20

3. Variant mutation in SARS-CoV-2 nucleocapsid enhances viral infection via altered genomic encapsidation;2024-03-11

4. An RNA replicon system to investigate promising inhibitors of feline coronavirus;Journal of Virology;2024-02-20

5. Classification, replication, and transcription of Nidovirales;Frontiers in Microbiology;2024-01-24