Affiliation:
1. Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061
Abstract
ABSTRACT
In
Escherichia coli
, gene products of the
glp
regulon mediate utilization of glycerol and
sn
-glycerol 3-phosphate. The
glpFKX
operon encodes glycerol diffusion facilitator, glycerol kinase, and as shown here, a fructose 1,6-bisphosphatase that is distinct from the previously described
fbp
-encoded enzyme. The purified enzyme was dimeric, dependent on Mn
2+
for activity, and exhibited an apparent
K
m
of 35 μM for fructose 1,6-bisphosphate. The enzyme was inhibited by ADP and phosphate and activated by phosphoenolpyruvate.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
94 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献