Substrate Ambiguity of 3-Deoxy- d - manno -Octulosonate 8-Phosphate Synthase from Neisseria gonorrhoeae Revisited-Reference-Cited by-同舟云学术

Substrate Ambiguity of 3-Deoxy- d - manno -Octulosonate 8-Phosphate Synthase from Neisseria gonorrhoeae Revisited

Published:2000-09 Issue:17 Volume:182 Page:5005-5008
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Sheflyan Galina Ya¹,Sundaram Appavu K.¹,Taylor William P.¹,Woodard Ronald W.¹

Affiliation:

1. Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065

Abstract

ABSTRACT Homogeneous, recombinant 3-deoxy- d - manno -octulosonate 8-phosphate synthase from Neisseria gonorrhoeae is shown to catalyze the formation of 3-deoxy- d - manno -octulosonate 8-phosphate from phosphoenolpyruvate and d -arabinose 5-phosphate as determined from 1 H-nuclear magnetic resonance analysis of the product. This enzyme does not catalyze the condensation of d -erythrose 4-phosphate and phosphoenolpyruvate to form 3-deoxy- d - ribo -heptulosonate 7-phosphate, as was previously reported (P. S. Subramaniam, G. Xie, T. Xia, and R. A. Jensen, J. Bacteriol. 180:119–127, 1998).

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.17.5005-5008.2000

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