Altered Stationary-Phase Response in a Borrelia burgdorferi rpoS Mutant-Reference-Cited by-同舟云学术

Altered Stationary-Phase Response in a Borrelia burgdorferi rpoS Mutant

Published:2000-05-15 Issue:10 Volume:182 Page:2909-2918
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Elias Abdallah F.¹,Bono James L.¹,Carroll James A.²,Stewart Philip¹,Tilly Kit¹,Rosa Patricia¹

Affiliation:

1. Laboratory of Human Bacterial Pathogenesis1 and

2. Microscopy Branch,2 Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

Abstract

ABSTRACT The homolog of the chromosomally encoded stationary-phase sigma factor RpoS in Borrelia burgdorferi was inactivated using gyrB r as a selectable marker. Two-dimensional nonequilibrium pH gradient electrophoresis of stationary-phase cell lysates identified at least 11 differences between the protein profiles of the rpoS mutant and wild-type organisms. Wild-type B. burgdorferi had a growth phase-dependent resistance to 1 N NaCl, similar to the stationary-phase response reported for other bacteria. The B. burgdorferi rpoS mutant strain was less resistant to osmotic stress in stationary phase than the isogenic rpoS wild-type organism. The results indicate that the B. burgdorferi rpoS homolog influences protein composition and participates in stationary-phase-dependent osmotic resistance. This rpoS mutant will be useful for studying regulation of gene expression in response to changing environmental conditions.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.10.2909-2918.2000

Reference60 articles.

1. Asbrink E. Hovmark A. Early and late cutaneous manifestations in Ixodes -borne borreliosis (erythema migrans borreliosis Lyme borreliosis) Lyme disease and related disorders. Benach J. L. Bosler E. M. 1988 4 15 New York Academy of Sciences New York N.Y

2. Isolation and cultivation of Lyme disease spirochetes;Barbour A. G.;Yale J. Biol. Med.,1984

3. Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody

4. Chronic Lyme borreliosis in the laboratory mouse;Barthold S. W.;Am. J. Pathol.,1993

5. Oligopeptide permease in Borrelia burgdorferi: putative peptide-binding components encoded by both chromosomal and plasmid loci;Bono J. L.;Microbiology,1998

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