Selective Kinase Inhibition Shows That Bur1 (Cdk9) Phosphorylates the Rpb1 Linker In Vivo-Reference-Cited by-同舟云学术

Selective Kinase Inhibition Shows That Bur1 (Cdk9) Phosphorylates the Rpb1 Linker In Vivo

Published:2019-08 Issue:15 Volume:39 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Chun Yujin¹²,Joo Yoo Jin¹²,Suh Hyunsuk¹²,Batot Gaëlle¹²,Hill Christopher P.¹²,Formosa Tim¹²,Buratowski Stephen¹²

Affiliation:

1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA

2. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA

Abstract

Cyclin-dependent kinases play multiple roles in RNA polymerase II transcription. Cdk7/Kin28, Cdk9/Bur1, and Cdk12/Ctk1 phosphorylate the polymerase and other factors to drive the dynamic exchange of initiation and elongation complex components over the transcription cycle. We engineered strains of the yeast Saccharomyces cerevisiae for rapid, specific inactivation of individual kinases by addition of a covalent inhibitor.

Funder

HHS | NIH | National Institute of General Medical Sciences

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.00602-18

Reference44 articles.

1. A chemical switch for inhibitor-sensitive alleles of any protein kinase

2. Structural Bioinformatics-Based Design of Selective, Irreversible Kinase Inhibitors

3. A Coupled Chemical-Genetic and Bioinformatic Approach to Polo-like Kinase Pathway Exploration