Analysis by Enzyme-Linked Immunosorbent Assay and PCR of Human Liver Abscess Aspirates from Patients in China for Entamoeba histolytica-Reference-Cited by-同舟云学术

Analysis by Enzyme-Linked Immunosorbent Assay and PCR of Human Liver Abscess Aspirates from Patients in China for Entamoeba histolytica

Published:1999-09 Issue:9 Volume:37 Page:3034-3036
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zengzhu G.¹²,Bracha R.¹,Nuchamowitz Y.¹,Cheng-I W.²,Mirelman D.¹

Affiliation:

1. Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel,1 and

2. Beijing Tropical Medicine Research Institute, Beijing, People’s Republic of China2

Abstract

ABSTRACT Aspirates of liver abscesses were analyzed for Entamoeba histolytica . PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) revealed that abscesses were caused by various strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.9.3034-3036.1999

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