CENTA as a Chromogenic Substrate for Studying β-Lactamases-Reference-Cited by-同舟云学术

CENTA as a Chromogenic Substrate for Studying β-Lactamases

Published:2001-06 Issue:6 Volume:45 Page:1868-1871
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Bebrone Carine¹,Moali Catherine¹,Mahy Florence¹,Rival Sandrine²,Docquier Jean Denis³,Rossolini Gian Maria³,Fastrez Jacques⁴,Pratt Rex F.⁵,Frère Jean-Marie¹,Galleni Moreno¹

Affiliation:

1. Centre for Protein Engineering, University of Liège, Institut de Chimie B6, Sart Tilman, 4000 Liège,1 and

2. Fachrichtung 8.8 Biochemie, Universität des Saarlandes, D-66123 Saarbrücken, Germany2;

3. Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy3; and

4. Laboratoire de Biochimie Physique et des Biopolymeres, Université Catholique de Louvain, Bâtiment Lavoisier, 1348 Louvain-la-Neuve,4 Belgium;

5. Department of Chemistry, Wesleyan University, Middletown, Connecticut 064595

Abstract

ABSTRACT CENTA, a chromogenic cephalosporin, is readily hydrolyzed by β-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of β-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.45.6.1868-1871.2001

Reference23 articles.

1. Technique for a rapid and efficient purification of the SHV-1 and PSE-2 beta-lactamases.;Bouillenne F.;J. Chromatogr. B Biomed. Sci. Appl.,2000

2. The 3-D structure of a zinc metallo-beta-lactamase from Bacillus cereus reveals a new type of protein fold.;Carfi A.;EMBO J.,1995

3. Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and dd-peptidases.;De Meester F.;Biochem. Pharmacol.,1987

4. Mechanism of inhibition of the PC1 beta-lactamase of Staphylococcus aureus by cephalosporins: importance of the 3′-leaving group.;Faraci W. S.;Biochemistry,1985

5. Purification and Biochemical Characterization of the VIM-1 Metallo-β-Lactamase

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