Affiliation:
1. Pharmacia Corporation, Kalamazoo, Michigan 49007
Abstract
ABSTRACT
The promoter for the
Staphylococcus aureus
capsule polysaccharide synthesis gene (
cap1A
) was cloned in front of the firefly luciferase gene for use in a cell extract
S. aureus
transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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