Affiliation:
1. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA
Abstract
ABSTRACT
With the enormous sizes viral populations reach, many variants are at too low a frequency to be detected by conventional next-generation sequencing (NGS) methods. Circular sequencing (CirSeq) is a method by which the error rate of next-generation sequencing is decreased so that even low-frequency viral variants can be accurately detected. The ability to visualize almost the entire genetic makeup of a viral swarm has implications for epidemiology, viral evolution, and vaccine design. Here we discuss experimental planning, analysis, and recent insights using CirSeq.
Funder
HHS | NIH | National Institute of Allergy and Infectious Diseases
University of California
DOD | Defense Advanced Research Projects Agency
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
16 articles.
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