Development of Microsphere-Based Multiplex Branched DNA Assay for Detection and Differentiation of Avian Influenza Virus Strains

Author:

Cha Wonhee12,Ma Yunqing3,Saif Yehia Mo12,Lee Chang-Won12

Affiliation:

1. Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691

2. Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210

3. Affymetrix, Inc., 6519 Dumbarton Circle, Fremont, California 94555

Abstract

ABSTRACT We developed and evaluated a multiplex branched DNA assay for the detection and subtyping of avian influenza (AI) virus strains. The assay successfully detected all 94 AI virus strains of 15 different hemagglutinin (HA) subtypes tested while simultaneously differentiating 24 North American H5, 11 Eurasian H5, and 11 H7 strains. Our study demonstrates for the first time that a branched DNA method can detect targets that show a great amount of sequence variation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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