Affiliation:
1. Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-1093
Abstract
ABSTRACT
We describe the construction of TnFuZ, a genetic tool for the discovery and mutagenesis of proteins exported from gram-positive bacteria. This tool combines a transposable element (Tn
4001
) of broad host range in gram-positive bacteria and an alkaline phosphatase gene (
phoZ
) derived from a gram-positive bacterium that has been modified by removal of the region encoding its export signal. Mutagenesis of
Streptococcus pyogenes
with TnFuZ (“FuZ” stands for fusions to
phoZ
) identified genes encoding secreted proteins whose expression was enhanced during growth in an aerobic environment. Thus, TnFuZ should be valuable for analysis of protein secretion, gene regulation, and virulence in gram-positive bacteria.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
25 articles.
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