Affiliation:
1. Pathology Department, The Methodist Hospital Research Institute, Houston, Texas
2. Mycobacteriology Department, Pasteur Institute of Iran, Tehran, Iran
3. Clinical Microbiology Department, Belarusian Research Institute of Epidemiology and Microbiology, Minsk, Belarus
Abstract
ABSTRACT
The aim of this study was to investigate the significance of multiple mutations in the
rpoB
gene as well as predominant nucleotide changes and their correlation with high levels of resistance to rifampin (rifampicin) in
Mycobacterium tuberculosis
isolates that were randomly collected from the sputa of 46 patients with primary and secondary cases of active pulmonary tuberculosis from the southern region (Afghanistan border) of Iran where tuberculosis is endemic. Drug susceptibility testing was performed using the CDC standard conventional proportional method. DNA extraction,
rpoB
gene amplification, and DNA sequencing analysis were performed. Thirty-five (76.09%) isolates were found to have multiple mutations (two to four) in the
rpoB
(β-subunit) gene. Furthermore, we demonstrate that the combination of mutations with more prevalent nucleotide changes were observed in codons 523, 526, and 531, indicating higher frequencies of mutations among patients with secondary infection. In this study, 76.08% (
n
= 35) of all isolates found to have mutation combinations involving nucleotide changes in codons 523 (GGG→GCG), 531 (TCG→TTG or TTC), and 526 (CAC→CGC, TTC, AAC, or CAA) demonstrated an association with higher levels of resistance to rifampin (MIC, ≥100 μg/ml).
Publisher
American Society for Microbiology
Reference37 articles.
1. Bakonyte, D., A. Baranauskaite, J. Cicenaite, A. Sosnovskaja, and P. Stakenas. 2005. Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis clinical isolates from Lithuania. Int. J. Tuberc. Lung Dis.9:936-938.
2. Molecular Characterization of Rifampin-Resistant Isolates of
Mycobacterium tuberculosis
from Hungary by DNA Sequencing and the Line Probe Assay
3. Physiological Cost of Rifampin Resistance Induced In Vitro in
Mycobacterium tuberculosis
4. Bobadilla-del-Valle, M., A. Ponce-de-Leon, C. Arenas-Huertero, G. Vargas-Alarcon, M. Kato-Maeda, P. M. Small, P. Couary, G. M. Ruiz-Palacios, and J. Sifuentes-Osornio. 2001. rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism. Emerg. Infect. Dis.7:1010-1013.
5. Rapid Detection of
rpoB
Gene Mutations in Rifampin-Resistant
Mycobacterium tuberculosis
Isolates in Shanghai by Using the Amplification Refractory Mutation System
Cited by
32 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献