Suppressor Mutations in LptF Bypass Essentiality of LptC by Forming a Six-Protein Transenvelope Bridge That Efficiently Transports Lipopolysaccharide-Reference-Cited by-同舟云学术

Suppressor Mutations in LptF Bypass Essentiality of LptC by Forming a Six-Protein Transenvelope Bridge That Efficiently Transports Lipopolysaccharide

Published:2023-02-28 Issue:1 Volume:14 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Falchi Federica A.¹,Taylor Rebecca J.²,Rowe Sebastian J.²,Moura Elisabete C. C. M.¹,Baeta Tiago³,Laguri Cedric³,Simorre Jean-Pierre³,Kahne Daniel E.²⁴,Polissi Alessandra¹,Sperandeo Paola¹^ORCID

Affiliation:

1. Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy

2. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA

3. University of Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France

4. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA

Abstract

The presence of an external LPS layer in the outer membrane makes Gram-negative bacteria intrinsically resistant to many antibiotics. Millions of LPS molecules are transported to the cell surface per generation by the Lpt molecular machine made, in E. coli , by seven essential proteins.

Funder

EC | Horizon Europe | Excellent Science | HORIZON EUROPE Marie Sklodowska-Curie Actions

HHS | NIH | OSC | Common Fund

Ministero dell'Istruzione, dell'Università e della Ricerca

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.02202-22

Reference41 articles.

1. The Bacterial Cell Envelope