Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence

Author:

Falah M1,Gupta R S1

Affiliation:

1. Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.

Abstract

The genes for hsp70 (or dnaK) have been cloned and sequenced from Rhizobium meliloti and Pseudomonas cepacia, two bacterial species belonging to the alpha- and beta-subdivisions of gram-negative proteobacteria, respectively. On the basis of global alignment of HSP70 proteins, several sequence signatures have been identified that are distinctive of mitochondrial homologs and gram-negative proteobacteria on the one hand and the chloroplasts and cyanobacteria on the other. Detailed phylogenetic analyses of HSP70 sequences from various eubacteria and eukaryotic organellar and cytosolic homologs support the inference regarding the origin of mitochondria from a member of the alpha-proteobacteria and of chloroplasts from cyanobacteria. The analysis presented here also suggests a monophyletic origin of the mitochondrial homologs.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

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3. Felsenstein J. 1993. PHYLIP manual version 3.5. Herbarium. University of Washington Seattle Wash.

4. Cloning of HSP70 gene from Clostridium perfringens using a general polymerase chain reaction based approach;Galley K. A.;Biochim. Biophys. Acta,1992

5. Organelle origins and ribosomal RNA;Gray M. W.;Biochem. Cell Biol.,1988

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