Affiliation:
1. Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Abstract
Two new loci, irrB and irrI, have been identified in Deinococcus radiodurans. Inactivation of either locus results in a partial loss of resistance to ionizing radiation. The magnitude of this loss is locus specific and differentially affected by inactivation of the uvrA gene product. An irrB uvrA double mutant is more sensitive to ionizing radiation than is an irrB mutant. In contrast, the irrI uvrA double mutant and the irrI mutant are equally sensitive to ionizing radiation. The irrB and irrI mutations also reduce D. radiodurans resistance to UV radiation, this effect being most pronounced in uvrA+ backgrounds. Subclones of each gene have been isolated, and the loci have been mapped relative to each other. The irrB and irrI genes are separated by approximately 20 kb of intervening sequence that encodes the uvrA and pol genes.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference29 articles.
1. Cloning of the DNA repair genes mtcA, mtcB, uvsD, uvsE, and the leuB gene from Deinococcus radiodurans;Al-Bakri G.;Gene,1985
2. Studies on a radio-resistant micrococcus. I. Isolation, morphology, cultural characteristics and resistance to gamma radiation;Anderson A. W.;Food Technol.,1956
3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
4. The control of post-irradiation DNA breakdown in Micrococcus radiodurans;Dean C. J.;Biochem. Biophys. Res. Commun.,1970
5. Molecular analysis of a mutant defective in photosynthetic oxygen evolution and isolation of a complementing clone by a novel screening procedure;Dzelzkalns V. A.;EMBO J.,1988