Characterization of a plasmid from the ruminal bacterium Selenomonas ruminantium

Author:

Martin S A1,Dean R G1

Affiliation:

1. Department of Animal and Dairy Science, The University of Georgia, Athens, 30602.

Abstract

A 4.8-kilobase-pair plasmid was isolated from the ruminal bacterium selenomonas ruminantium HD4 by using a sodium carbonate-EDTA washing buffer to improve cell lysis (R.G. Dean, S.A. Martin, and C. Carver, Lett. Appl. Microbiol. 8:45-48, 1989). This plasmid, designated pSR1, appears to be quite stable. No evidence of plasmid DNA was detected in S. ruminantium D or GA192. All three strains were tested for antibiotic resistance, and all were kanamycin resistant (MIC, 25 to 50 micrograms/ml). Only strain D was tetracycline resistant (MIC, 25 micrograms/ml), and all strains were sensitive to ampicillin (MIC, 1 microgram/ml). pSR1 was cloned into pBR322, and a map of pSR1 was constructed by using HindIII, ClAI, BamHI, and PvuII. Although ClaI, BamHI, ScaI, and EcoRV digested recombined plasmid isolated from Escherichia coli, these restriction endonucleases were not effective in digesting plasmid isolated directly from S. ruminantium HD4.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference18 articles.

1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979

2. The characteristics of strains of Selenomonas isolated from bovine rumen contents;Bryant M. P.;J. Bacteriol.,1956

3. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria;Caldwell D. R.;Appl. Microbiol.,1966

4. Carruthers M. M. 1985. Antimicrobial therapy p. 800-820. In G. P. Youmans P. Y. Paterson and H. M. Sommers (ed.) The biologic and clinical basis of infectious diseases 3rd ed. The W. B. Saunders Co. Philadelphia.

5. Prospects for the genetic manipulation of lactobacilli;Chassy B. M.;FEMS Microbiol. Rev.,1987

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