Affiliation:
1. Department of Genetics, University of Delhi South Campus, New Delhi 110021, India
Abstract
Zinc resistance in
Pseudomonas
sp. strain UDG26 was inducible. Induction led to enhanced uptake of the metal. A zinc-sensitive variant (UDG86) took up significantly less metal ion than the resistant one did. The affinity of uninduced and sensitive cells to zinc was less than that of resistant, induced cells. Metal accumulation by induced cells was not inhibited by azide, while 2,4-dinitrophenol and
N-N′
-dicyclohexylcarbodiimide enhanced zinc uptake because of inhibition of efflux. Transcription and translation inhibitors drastically reduced zinc accumulation, bringing it to the level found in the sensitive strain. These results suggest the involvement of protein(s) in zinc resistance.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference19 articles.
1. Bhagat R. and S. Srivastava. 1993. Biorecovery of zinc by Pseudomonas stutzeri RS34 p. 209-217. In A. E. Torma M. L. Apel and C. L. Brierly (ed.) Biohydrometallergical technologies vol. II. TMS Publications Warrendale Pa.
2. Energy-dependent zinc transport in Escherichia coli;Bucheder F.;Eur. J. Biochem.,1974
3. Cadmium transport by a Cd 2+-sensitive and a Cd 2+-resistant strain of Bacillus subtilis;Burke B. E.;Can. J. Microbiol.,1986
4. Accumulation and storage of Zn 2+ by Candida utilis;Failla M. L.;J. Gen. Microbiol.,1976
5. Gadd G. M. 1992. Heavy metal pollutants: environmental and biotechnological aspects p. 351-360. In J. Lederberg (ed.) Encyclopedia of microbiology vol. 2. Academic Press Inc. San Diego Calif.
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