Rapid Detection of Shigella Species in Environmental Sewage by an Immunocapture PCR with Universal Primers-Reference-Cited by-同舟云学术

Rapid Detection of Shigella Species in Environmental Sewage by an Immunocapture PCR with Universal Primers

Published:2002-05 Issue:5 Volume:68 Page:2580-2583
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Peng Xuanxian¹,Luo Wen¹,Zhang Jianying¹,Wang Sanying¹,Lin Shengcai¹

Affiliation:

1. The Key Laboratory of Education Ministry for Cell Biology & Tumor Cell Engineering, Department of Biology, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China

Abstract

ABSTRACT In the present study, we developed a quick, highly specific method for detection of Shigella species by combining immunocapturing of the bacteria and a universal primer PCR. The method drastically enhances test sensitivity, and it can be used not only for identification of Shigella species in the environment but also for rapid detection of other pathogens.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.5.2580-2583.2002

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