Affiliation:
1. Institute for Microbiology, Federal Dairy Research Centre, 24103 Kiel, Germany
Abstract
ABSTRACT
An efficient transformation protocol for
Gluconobacter oxydans
and
Acetobacter liquefaciens
strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the
Escherichia coli lacZY
genes in
G. oxydans
and
A. liquefaciens.
Although both
lac
genes were functionally expressed in both acetic acid bacteria
,
only a few transformants were able to grow on lactose. However, this ability strictly depended on the presence of a plasmid expressing both
lac
genes. Mutations in the plasmids and/or in the chromosome were excluded as the cause of growth ability on lactose.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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