Enzyme-linked immunosorbent assay for detection of antibody to the hepatitis B surface antigen-associated delta antigen

Author:

Crivelli O,Rizzetto M,Lavarini C,Smedile A,Gerin J L

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) was developed for specific detection of antibody to the hepatitis B surface antigen-associated delta antigen. The sensitivity of ELISA was intermediate between that of previously described immunofluorescence and radioimmunological assays for anti-delta. Performance of ELISA was simple and required only ordinary and inexpensive laboratory equipment.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

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2. Distribution of antibodies against the delta antigen in HBsAg carriers detected by a simple immunofluorescence blocking test;Crivelli O.;Acta Gastro-Enterol. Belg.,1978

3. Biophysical characterization of the adr subtype of hepatitis B antigen and preparation of anti-r sera in rabbits;Gerin J. L.;J. Immunol.,1975

4. Purification of naked intranuclear particles from human liver infected by hepatitis B virus;Hirshman Z. S.;Proc. Natl. Acad. Sci. U.S.A.,1974

5. Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay;Mathiesen L. R.;J. Clin. Microbiol.,1978

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