Affiliation:
1. Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan
Abstract
ABSTRACT
N
2
O reductase activity in soybean nodules formed with
Bradyrhizobium japonicum
was evaluated from N
2
O uptake and conversion of
15
N-N
2
O into
15
N-N
2
. Free-living cells of USDA110 showed N
2
O reductase activity, whereas a
nosZ
mutant did not. Complementation of the
nosZ
mutant with two cosmids containing the
nosRZDFYLX
genes of
B. japonicum
USDA110 restored the N
2
O reductase activity. When detached soybean nodules formed with USDA110 were fed with
15
N-N
2
O, they rapidly emitted
15
N-N
2
outside the nodules at a ratio of 98.5% of
15
N-N
2
O uptake, but nodules inoculated with the
nosZ
mutant did not. Surprisingly, N
2
O uptake by soybean roots nodulated with USDA110 was observed even in ambient air containing a low concentration of N
2
O (0.34 ppm). These results indicate that the conversion of N
2
O to N
2
depends exclusively on the respiratory N
2
O reductase and that soybean roots nodulated with
B. japonicum
carrying the
nos
genes are able to remove very low concentrations of N
2
O.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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