Broadly active inhibitor of viruses spontaneously produced by many cell types in culture

Author:

Baron S,McKerlie L

Abstract

A broadly active inhibitor of viruses (vaccinia, polio 1, and vesicular stomatitis) was found in the culture fluid from many types of normal human and mouse cells in culture. Virus plaque-inhibiting activity appeared in culture fluids within a few hour after incubation of cultures with fresh medium. Peak inhibitory activity occurred within 24 h. Blockade of cellular ribonucleic acid or protein synthesis decreased appearance of the inhibitor, thereby substantiating that it is a cell-produced viral inhibitor. Inhibition of virus required the simultaneous presence of inhibitor, virus, and cells (due to the reversible nature of the inhibition of virus attachment and penetration, as shown in the accompanying paper [T. K. Hughes et al., Infect Immun. 32:454-457, 1981]). The degree of inhibitory activity depended on the animal species of origin of the inhibitor, the cell type used for assay, and the virus type used for challenge. No cell species barrier against inhibitor action was found. Strong inhibition of multicycle yields of vesicular stomatitis virus and Sindbis virus was caused by low doses of inhibitor. These specific characteristics of the present inhibitor separate it from commonly recognized inhibitors. Possible biological significance of the inhibitor is discussed.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference10 articles.

1. General considerations of the interferon system;Baron S.;Texas Rep. Biol. Med.,1977

2. Antiviral action of mouse interferon in heterologous cells;Buckler C. E.;J. Bacteriol.,1966

3. A microplaque reduction assay for human and mouse interferon;Campbell J. B.;Can. J. Microbiol.,1975

4. The mammalian cell-virus relationship. II. Adsorption, reception and eclipse of poliovirus;Holland J. J.;J. Exp. Med.,1959

5. Cell-produced viral inhibitor: possible mechanism of action and chemical composition;Hughes T. K.;Infect. Immun.,1981

Cited by 31 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3