Identification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods-Reference-Cited by-同舟云学术

Identification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods

Published:2009-04 Issue:4 Volume:47 Page:1082-1086
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Mehta Maitry S.¹,Paule Suzanne M.¹,Thomson Richard B.¹²,Kaul Karen L.¹²,Peterson Lance R.¹²

Affiliation:

1. NorthShore University HealthSystem, Evanston, Illinois

2. Feinberg School of Medicine, Northwestern University, Chicago, Illinois

Abstract

ABSTRACT We compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identify Staphylococcus species directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of the Staphylococcus isolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01850-08

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