Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients

Author:

Zipeto D1,Revello M G1,Silini E1,Parea M1,Percivalle E1,Zavattoni M1,Milanesi G1,Gerna G1

Affiliation:

1. Istituto di Genetica, Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Pavia, Italy.

Abstract

The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised patients. Of the 86 patients, 23 underwent clinical and virologic follow-up for HCMV infection. In parallel, blood samples were examined for viremia and antigenemia. Concordant results between PCR and assays for viremia and antigenemia were obtained on 124 positive and 110 negative samples, with an overall concordance of 79.8%, while 59 samples (most from patients with HCMV infection) were positive by PCR alone. PCR is a new powerful tool for detection of HCMV infections in blood samples from immunocompromised patients. However, its clinical significance appears to be restricted to the indication of a risk of reactivation of HCMV infection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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