Affiliation:
1. Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis, California
Abstract
Mycoplasma
colonies and
Mycoplasma
cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange.
Mycoplasma
colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid were destroyed by perchloric acid. Detection of
Mycoplasma
in smears of mastitic milk or smears of infected lymph nodes was not definitive because of the large amount of nonspecific ribonucleic acid-rich material present during inflammatory reactions.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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